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Bacterial canker: diagnostic platform for specific detection of pathogen

15/05/2020 - François-Xavier Branthôme
New dataset helps tomato growers reduce spread of bacterial canker

 Bacterial canker of tomato is a disease that can lead to significant losses in greenhouse and field production systems. This disease is caused by the bacterial pathogen Clavibacter michiganensis. The best way to control bacterial canker is by securing clean tomato seed and enforcing good sanitation practices. 

A group of plant pathologists, primarily based at the University of California, Davis, became interested in studying Clavibacter when extension agents brought in diseased samples. In particular, they wanted to develop a diagnostic platform that could specifically detect the tomato pathogen, as some strains of Clavibacter do not cause disease. Clavibacter michiganensis is a Gram-positive bacterial pathogen that proliferates in the xylem vessels of tomato, causing bacterial wilt and canker symptoms. Accurate detection is a crucial step in confirming outbreaks of bacterial canker and developing management strategies. A major problem with existing detection methods are false-positive and -negative results.
Stem canker and unilateral leaf wilt caused by Clavibacter michiganensis on tomato

Taking advantage of the huge advances in DNA sequencing over the last 10 years, these plant pathologists were able to identify genetic regions that can specifically detect the pathogen causing bacterial canker of tomato without giving false positives with non-pathogenic bacteria. They analysed the genomes of 37 different Clavibacter strains in order to identify genetic regions only present in Clavibacter michiganensis. Using this information, they were able to develop a diagnostic platform.

"This diagnostic platform was based on amplification of three targets: two specific for Clavibacter michiganensis and one positive control," explained plant pathologist Gitta Coaker. "This platform is available for use without restriction and should facilitate the distribution of clean tomato seed to growers."

In an article in Phytopathology, Coaker and her team published large genomic datasets enabling other organizations to develop Clavibacter michiganensis-specific detection assay. The adoption of this assay in the field will help tomato growers reduce the spread of bacterial canker of tomato through easier screening of infected plant and seed materials.

Some complementary data
Comparative Genomics to Develop a Specific Multiplex PCR Assay for Detection of Clavibacter michiganensis

Sources: EurekAlert!,

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